Department of Botany
03-Biomolecules
The four major classes of biological molecules
polymers | monomers |
carbohydrates (starch, cellulose) | sugars (glucose) |
proteins | amino acids (20 to choose from) |
lipids (fats or oils = triglycerides) | fatty acids (+ glycerol) |
nucleic acids (DNA, RNA) | nucleotides (sugar, phosphate, N-base) |
monomer vs. polymer
mer = unit
mono = one
poly = many
polymers are made many identical (starch) or similar (proteins) monomers
The monomers are joined by covalent bonds to make the polymers. Water
is released as the covalent bonds form = condensation reaction.
(When the polymers are broken down - or digested - to release the monomers,
water is added to break the covalent bond = hydrolysis reaction.)
Carbohydrates
Mainly made of C, H, O. 1 C : 2 H : 1 O [CH2O]
monosaccharides: glucose, fructose (two examples of hexoses)
disaccharides: sucrose
polysaccharides: starch, cellulose
Functions of carbohydrates:
1. Structural
Cell Wall: cellulose (40-60%
of plant cell walls)
hemicellulose
pectin
2. Storage of food (calories) in seeds, roots, stems, wood, bark, leaves
Starch: amylose and amylopectin
Sucrose
3. Transport of calories and carbon skeletons
Sucrose
Proteins
contain C, H, O, N, and some S (two of the 20 amino acids have S)
Polymers of amino acids
The covalent bond between amino acids is called a peptide bond. Therefore,
proteins are sometimes called polypeptides.
The 20 amino acids have the same basic format, differing only in what
is known as the R group.
H-bonds and covalent bonds between S atoms of the R groups give proteins
their shapes and functional properties.
Functions of proteins
1. Structural
The plant cell wall is 2-10%
protein.
Cellular membranes are 40-75%
protein.
2. Storage
Seeds, roots, stems (wood
and bark of woody plants): storage of calories and N
3. Enzymes
catalysts: speed up chemical
reactions but are not consumed or changed by the reaction
As enzymes, proteins are
responsible for all metabolic reactions and the synthesis of all of the
other molecules found in organisms (pigments, hormones, etc.)
Lipids
Hydrocarbons, nonpolar. Contain H, C, and just a hint of O.
Functions of lipids
1. Storage
triglycerides (triacylglycerides,
TAGs): glycerol (3 C sugar alcohol) + 3 fatty acids
the fatty acids can be saturated or unsaturated
fats = solid at RT; oils = liquid at RT
for calorie storage, are usually found just in seeds. Exceptions: fruit
of avocado, olive
2. Structural
phospholipids in cell membranes
glycerol + 2 fatty acids + phosphate
phosphate end is polar = hydrophilic
fatty acid end is non-polar = hydrophobic
spontaneously form a bilayer in water: two hydrophilic surfaces with a
hydrophobic interior
waxes
fatty acids + long chain alcohols
cutin: in cuticle, which covers above ground plant parts
suberin: in cell walls of the endodermis (roots, pine needles) and cork
cells (bark)
Nucleic Acids
contain C, H, O, N, P
Basic building block: nucleotide
5 C sugar (pentose): ribose or deoxyribose
phosphate
N-base: cytosine, guanine, adenine, thymine/uracil
important monomers (ATP) and dinucleotides (NAD+)
polymers: DNA (two nucleotide polymer strands held together by H-bonds)
and RNA (one nucleotide polymer strand)
Gene expression: DNA --> RNA --> protein
Organic Acids
intermediates in
many reaction pathways and cycles; more oxidized than sugars but more reduced
than CO2; acidity of plant vacuoles
Lignin
second most
common organic molecule after cellulose; reinforces cell wall, most notably in
tracheary elements and sclerenchyma cells; decay resistant
Porphyrins (tetrapyrroles)
Chlorophylls: complexed with Mg (magnesium)
Hemes (cytochromes, other heme-containing proteins):
complexed with Fe (iron)
In cytochromes, the Fe undergoes oxidation/reduction (Fe3+/Fe2+)
during electron transfer processes of the mitochondrial electron transport chain
and chloroplast Z-scheme.
Secondary Metabolites
primary metabolites
found in all plants
perform essential roles in development and metabolism
examples: protein amino acids, cell wall sugars, hormones, photosynthetic pigments
secondary metabolites
generally distributed among limited groups of plants (Family, Order, etc.)
specific functions are often unknown
chemical structures and synthetic pathways are often complex
three main groups of molecules: phenolics, terpenes, alkaloids
Molecule classes are not confined to secondary metabolites
terpene primary metabolites: β-carotene,
lutein, zeaxanthin, abscisic acid, gibberellins, brassinosteroids
terpene secondary metabolites: essential oils, saponins, rubber
roles of secondary metabolites in plants:
anti-herbivory agents
anti-microbial agents
attractants for pollinators and seed/fruit dispersers
Oxidation and Reduction
Metabolism is the exchange of matter and energy between an organism and its
environment. Chemically, metabolism will involve oxidation and reduction
reactions of organic molecules. The carbon atoms in organic compounds are
oxidized or reduced as metabolism takes place. In a strict chemical
definition, oxidation is the loss of electrons and reduction is the gain of
electrons. This is fairly obvious when we look at ions like Fe in heme because
of the changes to the Fe ion's charge. In practical terms, when
looking at carbon compounds during metabolism, watch the number of oxygen and
hydrogen atoms per carbon atom. For example, let's compare carbon dioxide
and a carbohydrate. Carbon dioxide (CO2) is a low energy form
of carbon, and the carbon atom in carbon dioxide is said to be in a highly
oxidized state. Carbohydrates, such as the simple sugar glucose (C6H12O6),
are high energy forms of carbon. The carbon atoms in glucose are said to
be reduced. Compare the number of oxygen atoms to carbon atoms in carbon
dioxide and glucose. In carbon dioxide, there are two oxygen atoms for the
one carbon atom. In glucose, there is one oxygen atom per carbon.
This indicates that the carbon in carbon dioxide is more oxidized than the
carbon in glucose. Also, because glucose has less oxygen per carbon than
carbon dioxide, the carbons in glucose are more reduced than the carbon in
carbon dioxide. Now look at the number of hydrogen atoms per carbon.
Carbon dioxide has no hydrogen atoms, while glucose has two hydrogens for each
carbon. Reduced carbon atoms have more hydrogen associated with them than
oxidized carbons do. How reduced are the carbon atoms in a typical fat,
like palmitic acid? The chemical formula is C16 H32O2.
There are two hydrogens per carbon, just like in glucose, but there is only one
oxygen atom for every eight carbon atoms. Fats contain very reduced
carbon. The more reduced a carbon atom is, the more energy (calories) it
contains. This is why fats contain more calories per C than carbohydrates
do; the carbon in fats is more reduced than the carbon in carbohydrates.
So when we look at matter and energy conversions during metabolism, we are
looking at oxidation and reduction of carbon atoms, with reduction putting
energy into carbon atoms (as happens during photosynthesis) and oxidation
releasing that energy (as happens during respiration).
Review
Condensation vs. Hydrolysis
Relate to the synthesis and breakdown of polymers in living
organisms
Carbohydrates
examples of monomers and polymers
What distinguishes carbohydrates structurally from other groups of
molecules?
What functions do carbohydrates and soluble sugars have in cells?
Lipids
examples
What distinguishes lipids structurally from other groups of
molecules?
What functions do lipids have in cells?
Proteins
monomers; peptide bond
primary, secondary, tertiary, and quaternary structure
What distinguishes proteins and amino acids structurally from
other groups of molecules?
What functions do proteins have in cells?
Nucleic Acids
examples of monomers, dimers, and polymers
What distinguishes nucleic acids structurally from other
groups of molecules?
What functions do nucleic acids and nucleotides have in cells?
Porphyrins
basic structure; complexes with a metal ion
function of chlorophylls (Mg = magnesium)
function of cytochromes (Fe = iron)
Oxidation vs. Reduction
Relate to carbon atoms in organic molecules and CO2
With regard to calories, what is the consequence of reduction of
C? Of oxidation?
Web Sites
http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookCHEM2.html#Organic%20molecules
http://arnica.csustan.edu/Biol1010/biochemistry/biochemistry.htm
http://web.mit.edu/esgbio/www/lm/lipids/lipids.html
http://web.mit.edu/esgbio/www/lm/sugars/sugars.html
http://web.mit.edu/esgbio/www/lm/proteins/aa/aminoacids.html
http://web.mit.edu/esgbio/www/lm/proteins/peptidebond.html
http://web.mit.edu/esgbio/www/lm/nucleicacids/nucleicacids.html
http://web.mit.edu/esgbio/www/lm/nucleicacids/dna.html
http://www.hcs.ohio-state.edu/hcs300/biochem1.htm
http://www.hcs.ohio-state.edu/hcs300/biochem2.htm
http://ntri.tamuk.edu/homepage-ntri/lectures/biology/lecture3.html
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25 August 2003; links checked 25 August 2003